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. 2024 Jun 6;7(8):e202302503. doi: 10.26508/lsa.202302503

Figure 5. STAT3 cytoplasmic translocation upon STAT3i treatment is coupled with the activation of autophagy in young and old muscle stem cells (MuSCs).

Figure 5.

(A) Schematic representation of MuSC isolation from 2 (young)- or 15 (old)-month-old WT mice not treated (NT) or treated with the STAT3i. MuSCs were isolated 3 d.p.i. (B) Representative images of LAMP1 (green), STAT3 (red), and DAPI (blue) immunostaining in young or old MuSCs NT or treated with the STAT3i. Arrows represent cells with cytoplasmic retention of STAT3 and active autophagy. Scale bar = 10 μm. (C) Plot represents the percentage of LAMP1+ MuSCs with a cytoplasmic versus nuclear STAT3 localization. (n = 3; 20 MuSCs per biological sample were counted; values represent the mean ± s.d., ***P < 0.001 by one-way ANOVA). (D) Plot represents the corrected total cell fluorescence of STAT3 nuclear over the total STAT3 (n = 3; 20 MuSCs per biological sample were counted; values represent the mean ± s.d., *P < 0.05 and ***P < 0.001 by one-way ANOVA).