FIG. 3.

MxA action depends on the site of nucleocapsid assembly. Cells were transfected with the components of the THOV minireplicon system as described in the legend to Fig. 1. The CAT minigenome was transcribed either with the nuclear RNA polymerase I (Pol I) by using pPolI-THOV/CAT (500 ng) (A) or with the cytoplasmic T7 polymerase by using the plasmid pT7ribo-THOV/CAT (100 ng) (B). Plasmid DNA (1 μg) encoding either wild-type MxA, MxA(T103A), or TMxA (a nuclear form of wild-type MxA containing a foreign nuclear localization signal) was added to the plasmid mixtures. The CAT/Luc ratio in the presence of 1 μg of pBSK(+) was set to 1 (control).