Table 2.
Secondary structures of whey protein isolate (WPI) in the absence and presence of lutein/zeaxanthin (Lut/Zx) under different pH conditions.
| Sample | pH | α-helix (%) | β-sheet (%) | Turn (%) | Random coil (%) |
|---|---|---|---|---|---|
| WPI | 2.5 | 18.5 ± 0.6 | 31.1 ± 0.1 | 31.7 ± 0.2 | 18.7 ± 0.7 |
| 5.5 | 18.6 ± 0.3 | 28.9 ± 0.4 | 34.7 ± 0.8 | 17.8 ± 1.1 | |
| 7.5 | 19.4 ± 0.4 | 24.9 ± 0.2 | 35.2 ± 0.5 | 20.5 ± 0.5 | |
| 9.5 | 22.3 ± 0.4 | 19.7 ± 0.3 | 37.5 ± 0.8 | 20.4 ± 0.9 | |
| WPI-Lut | 2.5 | 18.6 ± 0.3 | 31.2 ± 0.6 | 32.1 ± 0.2 | 18.1 ± 0.9 |
| 5.5 | 18.2 ± 0.4 | 28.7 ± 0.6 | 34.7 ± 0.2 | 18.4 ± 0.7 | |
| 7.5 | 20.2 ± 0.3 | 24.7 ± 0.8 | 35.1 ± 0.4 | 20.0 ± 0.3 | |
| 9.5 | 22.4 ± 0.9 | 19.1 ± 0.7 | 38.4 ± 0.6 | 20.1 ± 0.7 | |
| WPI-Zx | 2.5 | 18.7 ± 0.5 | 31.3 ± 0.4 | 31.9 ± 0.1 | 18.1 ± 0.3 |
| 5.5 | 19.5 ± 0.3 | 28.3 ± 0.4 | 33.6 ± 0.6 | 18.6 ± 0.1 | |
| 7.5 | 19.8 ± 0.3 | 22.5 ± 0.7 | 38.6 ± 0.1 | 19.1 ± 0.6 | |
| 9.5 | 23.1 ± 0.9 | 18.6 ± 0.2 | 38.0 ± 0.4 | 20.3 ± 0.5 |