| Antibodies |
|
| Mouse anti-β-Actin (8226) |
Abcam |
ab8226; RRID: AB_306371 |
| Rabbit anti-Biotin (D5A7) |
Cell Signaling |
5597S; RRID: AB_10828011 |
| Goat anti-Rabbit IgG, (H + L), HRP |
Cell Signaling |
7074; RRID: AB_2099233 |
| Horse anti-Mouse IgG, (H + L), HRP |
Cell Signaling |
7076; RRID: AB_330924 |
| Mouse anti-α1–7 proteasome subunit |
Enzo |
BML-PW8195; RRID: AB_2052368 |
| Rabbit anti-β5 proteasome subunit |
Enzo |
BML-PW8895; RRID: AB_10540901 |
| Rabbit anti-CGRP |
Immunostar |
24112; RRID: AB_572217 |
| Chicken anti-NF-H |
Millipore Sigma |
AB5539; RRID: AB_11212161 |
| Mouse anti-Na+/K+-ATPase |
Millipore Sigma |
05–369; RRID: AB_309699 |
| Mouse anti-βIII-tubulin |
Millipore Sigma |
T8578; RRID: AB_1841228 |
| IB4, biotin conjugate |
Millipore Sigma |
L2140; RRID: AB_2313663 |
| Rabbit anti-β2 |
Novus |
NBP1–92294; RRID: AB_1104294 |
| Donkey anti-Goat IgG, (H + L), HRP |
Promega |
V8051; RRID: AB_430838 |
| Goat anti-β2 |
Santa Cruz |
sc-54676; RRID:AB_2172024 |
| Goat anti-β5 |
Santa Cruz |
sc-55009; RRID: AB_2237739 |
| Goat anti-α2 |
Santa Cruz |
sc-54715; RRID: AB_2171410 |
| Rabbit anti-Chicken IgY (H + L), HRP |
Thermo Fisher Sci. |
31401; RRID: AB_228385 |
| Mouse anti-Transferrin receptor (H68.4) |
Thermo Fisher Sci. |
136800; RRID: AB_2533029 |
| Anti-P2X3 |
Thermo Fisher Sci. |
PA5–77682; RRID: AB_2736292 |
| Anti-goat-488 |
Thermo Fisher Sci. |
A32814TR; RRID: AB_2866497 |
| Rabbit anti-PGP9.5 |
Thermo Fisher Sci. |
PA5–16825; RRID: AB_10986012 |
| Rabbit anti-GFAP |
Thermo Fisher Sci. |
PA-110019; RRID: AB_1074611 |
|
| Biological samples |
|
| Mouse spinal cord, DRG, and nerve |
Charles River |
RRID: IMSR_CRL:27 |
| Mouse skin tissue |
Charles River |
RRID: IMSR_CRL:27 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Precision Plus Standards All Blue |
Bio-Rad |
1610393 |
| TEMED |
Bio-Rad |
1610800 |
| 40% Acrylamide/Bis Solution, 37.5:1 |
Bio-Rad |
1610148 |
| 30% Acrylamide/Bis Solution, 29:1 |
Bio-Rad |
1610156 |
| Phosphate Buffered Saline (PBS) |
Corning |
46–013-CM |
| Paraformaldehyde |
EMS |
15710 |
| Horse serum |
GE Lifesciences |
SH30074.03 |
| Fura-2-acetoxymethyl ester |
Invitrogen |
F1201 |
| Tris |
Millipore Sigma |
77–86-1 |
| Sodium deoxycholate |
Millipore Sigma |
264103 |
| DMSO |
Millipore Sigma |
D8418 |
| β-methylene ATP |
Millipore Sigma |
5054190001 |
| 5-Fluoro-2’ -deoxyuridine |
Millipore Sigma |
F0503 |
| Fetal calf serum (FCS) |
Millipore Sigma |
SH30071 |
| Fetal bovine serum (FBS) |
Millipore Sigma |
A9647 |
| Nerve growth factor (NGF) |
Millipore Sigma |
N0513 |
| Donkey serum |
Millipore Sigma |
566460 |
| Potassium Chloride (KCI) |
Millipore Sigma |
P5405 |
| Magnesium Chloride (MgCI2) |
Millipore Sigma |
M2393 |
| Calcium chloride (CaCI2) |
Millipore Sigma |
C7902 |
| Sorbitol |
Millipore Sigma |
56755-M |
| β-glycerophosphate |
Millipore Sigma |
G5422 |
| NaHCO3 |
Millipore Sigma |
S5761 |
| Glucose |
Millipore Sigma |
G7021 |
| Liberase TM |
Millipore Sigma |
5401119001 |
| Liberase TL |
Millipore Sigma |
5401020001 |
| Glycine |
Millipore Sigma |
4840 |
| Epoxomicin |
Millipore Sigma |
E3652 |
| cOmplete Protease inhibitor |
Millipore Sigma |
11836170001 |
| ECL Cytiva, RPN2106 |
Millipore Sigma |
Ref# GERPN2106 |
| Poly-L-Lysine |
Millipore Sigma |
P4707 |
| Adenosine triphosphate (ATP) |
Millipore Sigma |
A26209; CAS:34369–07-8 |
| Penicillin/streptomycin |
Millipore Sigma |
15140122 |
| Trypsin inhibitor |
Millipore Sigma |
T9253; CAS: 9035–81-8 |
| L-cysteine |
Millipore Sigma |
168149; CAS: 52–90-4 |
| Bovine Serum Albumin (BSA) |
Millipore Sigma |
A9647; CAS: 9048–46-8 |
| Glycerol |
Millipore Sigma |
G6279; CAS: 56–81-5 |
| Xylene cyanol FF |
Millipore Sigma |
335940; CAS: 2650–17-1 |
| Sodium dodecyl sulfate (SDS) |
Millipore Sigma |
L3771; CAS: 151–21-3 |
| Ammonium persulfate (APS) |
Millipore Sigma |
A3678; CAS: 7727–54-0 |
| Sodium chloride (NaCl) |
Millipore Sigma |
S9888; CAS: 7647–14-5 |
| Tween 20 |
Millipore Sigma |
P2287 |
| EDTA |
Millipore Sigma |
E5134; CAS: 6381–92-6 |
| Sodium orthovanadate |
Millipore Sigma |
567540 |
| HEPES buffer (1M), pH 7.3 |
Quality Biological |
118–089-721 |
| DPBSMC |
Quality Biological |
114–059-101 |
| Fluoromount-G |
Southern Biotech |
0100–01 |
| Tissue-Tek O.C.T. |
Sakura Finetek |
4583 |
| DMEM/F12 |
Thermo Fisher Sci. |
11320033 |
| Triton X-100 |
Thermo Fisher Sci. |
X100 |
| B27 |
Thermo Fisher Sci. |
17504044 |
| HOECHST |
Thermo Fisher Sci. |
H21492 |
| Hanks Balanced Salt Solution (HBSS) |
Thermo Fisher Sci. |
H9394; CAS: 14170112 |
| L-Glutamine |
Thermo Fisher Sci. |
25030081 |
| Dithiothreitol (DTT) |
Thermo Fisher Sci. |
R0861 |
| DMSO |
Thermo Fisher Sci. |
25–950-CQC |
| Sulfo-NHS-SS-biotin |
Thermo Fisher Sci. |
21335 |
| Neutravidin agarose |
Thermo Fisher Sci. |
29202 |
| DMEM |
Thermo Fisher Sci. |
11995065 |
| Papain |
Worthington |
Cat#:9001–73-4 |
| Biotin-epoxomicin |
Synthesized |
In-house |
| Bodipy TMR-Ahx3L3VS (MV151) |
Lab of Dr. Alexei F. Kisselev |
https://doi.org/10.1016/j.chembiol.2006.09.013
|
|
| Critical commercial assays |
|
| Quick Start Bradford Protein Assay |
Bio-Rad |
5000201 |
|
| Deposited Data |
|
| scRNA-Seq |
This paper |
GEO: GSE261727
|
|
| Experimental models: Organisms/strains |
|
| C57BL/6 |
Charles River |
RRID: IMSR_CRL:27 |
|
| Software and algorithms |
|
| ImageJ |
Schindelin et al.138
|
https://imagej.nih.gov/ij/
|
| GraphPad Prism |
GraphPad |
http://www.graphpad.com/
|
| Von Frey Up Down Method calculator |
Christensen et al.139
|
https://github.com/MikkelAStorm/Up.Down.Method
|
| NIS Elements imaging software |
Nikon |
https://www.microscope.healthcare.nikon.com/
|
| Seurat |
Hao et al.141
|
https://github.com/satijalab/seurat
|
| Harmony |
Korsunsky et al.142
|
https://github.com/immunogenomics/harmony
|
| Fgsea v1.24.0 |
Korotkevich et al.143
|
https://github.com/ctlab/fgsea
|
|
| Other |
|
| Gel Doc EZ System |
Bio-Rad |
1708270EDU |
| GE Typhoon FLA 9500 |
Cytiva |
N/A |
| Austerlitz pin |
Fine Science Tools |
000 |
| Von Frey filaments |
North Coast Medical |
NC12775–02 to NC12775–09 |
