TABLE 3.

Primers, annealing temperatures, PCR template, and methods of sequence determination for the gB of each macaque rhadinovirus isolate^a

Macaque rhadinovirus	Primers	Primer annealing temp (°C)	PCR template and method of sequence determination
RRV492-98	H3 and H4	58	PCR fragment, directly sequenced with specific primers
RRV309-95	H1 and H45	58	Cloned AluI and RsaI restriction fragments, sequenced with M13 forward and reverse primers, gaps filled with specific primers
CRV27-97	H1 and H46	50	Cloned AluI and RsaI restriction fragments, sequenced with M13 forward and reverse primers
CRV472-97	H1 and H5	50	Cloned AluI and RsaI restriction fragments, sequenced with M13 forward and reverse primers
CRV23-97	H1 and H5	50	Cloned AluI and RsaI restriction fragments, sequenced with M13 forward and reverse primers
PMRV98126	P2 and P3	58	PCR fragment, directly sequenced with specific primers
PMRV19545b	H1 and H5, H42 and H45	50	Cloned AluI and RsaI restriction fragments, sequenced with M13 forward and reverse primers, gaps filled with specific primers

^aThe sequence of each primer is shown in Table 2. 

^bAmplification of PMRV19545 gB sequences required nested PCR with the four primers shown.