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. 2024 Jun 7;23:124. doi: 10.1186/s12943-024-02016-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — PLGC cells with high OLFM4 expression displayed activation of the Wnt signaling pathway: a The GSEA of the GSE78523 RNA-seq dataset in Hallmark genes and KEGG was analyzed. b The Addmodulescore analysis of the scRNA-seq dataset GSE134520 was analyzed to exhibit the functions enriched in the PLGC cells with high OLFM4 expression. c-d Western Blotting confirmed that the relation between the expression of OLFM4 and the expression of molecules related to Wnt/β-catenin signaling pathway, EMT transition, and c-Myc. e-f HE staining and AB-PAS staining were conducted to diagnose intestinal metaplasia, while the confocal immunofluorescence was performed to show the expression of OLFM4, Ki67, E-cadherin and Vimentin