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. 2024 Jun 7;19:44. doi: 10.1186/s13062-024-00486-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — MCAM was overexpressed in OS tissues and cells. (A) The mRNA level of MCAM in OS tissues and normal tissues was detected by qRT-PCR. (B) The overall survival of OS patients in Low expression group and High expression group was analyzed. (C) The expression of MCAM in OS patients with positive or negative lymph node metastasis was determined by qRT-PCR. (D) The mRNA expression of MCAM in OS patients in TNM grades III or I+II was determined by qRT-PCR. (E) The protein level of MCAM in OS tissues and normal tissues was measured by western blot. (F) The protein level of MCAM in hFOB1.19, HOS, MG63 and U2OS cells was measured through western blot. *P < 0.05, **P < 0.01, ***P < 0.001