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. 2024 Jun 7;19:44. doi: 10.1186/s13062-024-00486-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Knockdown of MCAM inhibited cell growth, invasion and migration and facilitated apoptosis in OS cells. MG63 and U2OS cells were transfected with sh-NC or sh-MCAM. (A) MCAM protein level in transfected MG63 and U2OS cells was measured by western blot. (B) MG63 and U2OS cell viability was evaluated by MTT assay. (C) The colony formation ability of MG63 and U2OS cells was assessed by colony formation assay. (D) MG63 and U2OS cell apoptosis was analyzed by flow cytometry analysis. (E) The invasion of MG63 and U2OS cells was evaluated by transwell assay. (F) The migration of MG63 and U2OS cells was examined with wound-healing assay. (G and H) The protein levels of CyclinD1 and MMP9 in MG63 and U2OS cells were measured via western blot. **P < 0.01, ***P < 0.001