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. 2024 Jun 7;19:44. doi: 10.1186/s13062-024-00486-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effects of MCAM overexpression on OS cell proliferation, apoptosis, invasion and migration. MG63 and U2OS cells were transfected with MCAM or pcDNA. (A) MCAM protein level in MG63 and U2OS cells was determined via western blot. (B and C) The viability and colony formation of MG63 and U2OS cells were estimated by MTT assay and colony formation assay. (D and E) The apoptosis of MG63 and U2OS cells was analyzed by flow cytometry analysis. (F and G) The invasion and migration abilities of MG63 and U2OS cells were examined by transwell assay and wound-healing assay. (H and I) The protein levels of CyclinD1 and MMP9 in MG63 and U2OS cells were measured via western blot. **P < 0.01, ***P < 0.001