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. 2024 Jun 7;19:44. doi: 10.1186/s13062-024-00486-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — METTL3 targeted MCAM. (A) The m6A methylation modification sites of METTL3 in MCAM was predicted by RM2target database. (B) After MeRIP, MCAM enrichment was determined by qRT-PCR. (C) The protein level of METTL3 in MG63 and U2OS cells transfected with sh-NC or sh-METTL3 was measured via western blot. (D) MCAM m6A level in MG63 and U2OS cells transfected with sh-NC or sh-METTL3 was determined. (E) SRAMP predicted MCAM mRNA contained the m6A modification site of METTL1. (F and G) The interaction between MCAM and METTL3 was verified by dual-luciferase reporter assay. (H) The mRNA expression of METTL3 in OS tissues and normal tissues was determined by qRT-PCR. (I) The linear correlation between METTL3 mRNA level and MCAM mRNA level in OS tissues was estimated by Spearman’s coVrrelation coefficient analysis. (J and K) After treatment with Act D for 0 h, 3 h, 6 h and 9 h, MCAM mRNA expression was determined by qRT-PCR. (L) MCAM protein level in MG63 and U2OS cells with sh-NC or sh-METTL3 transfection was measured via western blot. (M) The binding association between MCAM and METTL3 was analyzed by Co-IP assay. *P < 0.05, **P < 0.01, ***P < 0.001