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. 2024 Jun 7;19:44. doi: 10.1186/s13062-024-00486-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — IGF2BP1 affected METTL3-mediated the m6A methylation modification of MCAM. (A) After sh-NC, sh-IGF2BP1, sh-IGF2BP2 or sh-IGF2BP3 transfection in MG63 and U2OS cells, MCAM protein level was measured by western blot. (B) The relation between IGF2BP1 and MCAM was predicted by ENCORI. (C) IGF2BP1 mRNA level in OS tissues and normal tissues was determined by qRT-PCT. (D) The linear correlation between the mRNA levels of IGF2BP1 and MCAM in OS tissues was analyzed. (E and F) The effect of METTL3 knockdown on the interaction between IFG2BP1 and MCAM was estimated by RIP assay. (G) The protein level of IGF2BP1 in MG63 and U2OS cells transfected with sh-NC, sh-IGF2BP1, pcDNA or IGF2BP1 was measured by western blot. (H and I) After Act D treatment, MCAM mRNA level in MG63 and U2OS cells transfected with sh-NC, sh-METTL3, sh-METTL3 + pcDNA or sh-METTL3 + IGF2BP1 was determined by qRT-PCR. (J) The effects of METTL3 and IGF2BP1 on MCAM m6A level in MG63 and U2OS cells transfected with sh-NC, sh-METTL3, sh-METTL3 + pcDNA or sh-METTL3 + IGF2BP1 were analyzed. (K) MCAM protein level in MG63 and U2OS cells transfected with sh-NC, sh-METTL3, sh-METTL3 + pcDNA or sh-METTL3 + IGF2BP1 was measured by western blot. *P < 0.05, **P < 0.01, ***P < 0.001