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. 2024 Jun 7;19:44. doi: 10.1186/s13062-024-00486-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — YY1 promoted the transcriptional regulation of METTL3 in OS cells. (A) Jaspar database predicted binding sites between transcription factor YY1 and METTL3 promoter. (B) ChIP assay was performed the combination between YY1 and METTL3. (C) The protein expression of YY1 in MG63 and U2OS cells transfected with si-NC, si-YY1, pcDNA or YY1 was determined by western blot. (D and E) The luciferase activity in MG63 with pcDNA/YY1 and WT-METTL3/MUT-METTL3 co-transfection and U2OS cells with si-NC/si-YY1 and WT-METTL3/MUT-METTL3 co-transfection was examined through dual-luciferase reporter assay. (F) METTL3 protein level in MG63 and U2OS cells transfected with si-NC, si-YY1, pcDNA or YY1 was measured via western blot. **P < 0.01, ***P < 0.001