Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jun 7;22:546. doi: 10.1186/s12967-024-05263-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Plasma exosomes were taken up by human OFs. (a-c) Characterization of Pla-Exos. (a) Western blot analysis identified positive expression of exosomes markers CD63, Alix and TSG101, with the negative expression of Golgi marker Calnexin in the extraction samples. (b) Nano-Sight analysis indicated that the size of the extraction was approximately 100 nm. (c) The morphology of the extraction was examined via transmission electron microscope. (d, e) Characterization of OFs. (d) Immunofluorescence staining of OFs reveals the uniform expression of the fibroblast markers FSP and vimentin. Cells were stained with anti-FSP and anti-vimentin antibodies and Alexa Fluor 488/594-conjugated secondary antibodies (fluorescence microscopy, 20; scale bars, 50 μm). (e) OFs were analyzed for Thy-1 expression using flow cytometry (right). The Thy-1⁺ subset was obtained from parental OFs after 2 rounds of magnetic bead sorting. Thy-1⁺ OFs were > 99% positive for Thy-1 (left). (f) Pla-Exos (green) were transferred into OFs (blue) (fluorescence microscopy, 20). FSP fibroblast surface protein, VIM vimentin