Table 1.

Sequences of γ‐synuclein gene‐specific primers

Primer		Sequence (5’‐3’)	Product size (bp)
RT‐PCR primers 17
γ‐synuclein‐RT‐5′		CAAGAAGGGCTTCTCCATCGCCAAGG	338 (60 to 397)
γ‐synuclein‐RT‐3′		CCTCTTTCTCTTTGGATGCCACACCC
GAPDH‐RT‐5′		GAAGGTGAAGGTCGGAGTC	226 (108 to 333)
GAPDH‐RT‐3′		GAAGATGGTGATGGGATTTC
Real‐time RT‐PCR primers
γ‐synuclein‐Real‐RT‐5′		GGAGGACTTGAGGCCATCTG	73 (339 to 411)
γ‐synuclein‐Real‐RT‐3′		CTCCTCTGCCACTTCCTCTTTC
GAPDH‐Real‐RT‐5′		GGACCTGACCTGCCGTCTAG	100 (831 to 930)
GAPDH‐Real‐RT‐3′		GTAGCCCAGGATGCCCTTGA
Bisulfite sequencing PCR primers 18
γ‐synuclein‐SF		GGTTGAGTTAGTAGGAGTTTA	415 (−275 to +140)
γ‐synuclein‐SR		CCTACCATACCCCACTTACCC
Nested MSP primers 18
Methylated	γ‐synuclein‐MF	TCGTATTAATATTTTATCGGCGT	194 (−139 to +55)
	γ‐synuclein‐MR	CCGCACCCACCACGCCCTCCTTAACGA
Unmethylated	γ‐synuclein‐UF	TTGGTGTTAATAGGAGGTATTGGGGATAGTTGTTGTG	177 (−123 to +54)
	γ‐synuclein‐UR	CACACCCACCACACCCTCCTTAACAAT
Real‐time Q‐MSP primers
Methylated	γ‐synuclein‐Real‐MF	TTCGTATTAATATTTTATCGGCGT	104 (−140 to −37)
	γ‐synuclein‐Real‐MR	GAAACTAAATCTCCCTACGAACTACG
Unmethylated	γ‐synuclein‐Real‐UF	GGTTTTTGTATTAATATTTTATTGGTG	110 (−144 to −35)
	γ‐synuclein‐Real‐UR	ACAAAACTAAATCTCCCTACAAACTACAA
WT primers 18
	γ‐synuclein‐WF	ACGCAGGGCTGGCTGGGCTCCA	242 (−169 to +73)
	γ‐synuclein‐WR	CCTGCTTGGTCTTTTCCACC

The CpG sites within the primer sequences are underlined