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. 2009 Mar 9;100(4):759–769. doi: 10.1111/j.1349-7006.2009.01096.x

Figure 4.

Figure 4

Forced expression of Noxa with viral‐free vectors. (a) The polymeric micelle vector including HA‐Noxa, HA‐Puma cDNA, or empty was injected intravenously into tumor‐transplanted mice and then the tumor size was measured. The experiments were carried out in quadruplicate and the results are presented as the mean ± SD. (b–f) Histopathological examinations of micelle vector‐injected tumor and the surrounding tissue. Micelle vectors contained cDNA of (b,e) HA‐Noxa, (c,f) HA‐Puma, and (d) empty. The sliced tumors and tissues were stained with hematoxylin–eosin (HE), anti‐HA antibody, or TdT‐mediated dUTP‐biotin nick‐end labeling (TUNEL). White bars = 200 µm, black bars = 50 µm. Induction of apoptotic cell death in micelle vectors used to treat (g) tumors and (h) surrounding tissue. The rates of TUNEL‐positive and HA‐positive cells were measured in 10 independent fields. The results are presented as the mean + SD.