Table 1.

PCR primers used for real‐time quantitative RT‐PCR analysis

Gene	Primer	Product size (bp)
GAPDH	F: 5′‐AACGGCACAGTCAAGGCTGAGAACG‐3′ R: 5′‐CAACATACTCGGCACCGGCATCG‐3′	118
IL‐1β	F: 5′‐TGACTTCACCTTGGAATCCGTCTCT‐3′ R: 5′‐GGCAACAAGGGAGCTCCATCAC‐3′	91
TNF‐α	F: 5′‐GCTGCCCCCACCTCGTGCTC‐3′ R: 5′‐CTTGATGGCAGACAGGAGGCTGACC‐3′	89
COX‐2	F: 5′‐GCCGTCGAGTTGAAAGCCCTCTACA‐3′ R: 5′‐CCCCGAAGATGGCGTCTGGAC‐3′	97
iNOS	F: 5′‐GCATGACCTTGGTGTTTGGGTGCC‐3′ R: 5′‐GCAGCCTGTGTGAACCTGGTGAAGC‐3′	110

GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IL‐1β, interleukin‐1β; TNF‐α, tumor necrosis factor‐α; COX‐2, cyclooxygenase‐2; iNOS, inducible nitric oxide synthase; F, forward; R, reverse.