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. 2007 Apr 12;98(7):943–948. doi: 10.1111/j.1349-7006.2007.00480.x

Figure 2.

Figure 2

Results of the methylation analysis of the 5′ upstream region of rat Tslc1 (tumor suppressor in lung cancer 1) gene. (a) For each sample, eight clones were sequenced. The primer pair for bisulfite sequencing is shown (arrowheads). There is a CpG site within the primer sequence of BS‐R. Methylated CpG sites are shown by closed circles and unmethylated CpG sites are shown by open circles. Reduced expression of Tslc1 was seen in HCC4, 5 and 8, but not in NRL1 and HCC1. NRL, normal liver tissue, HCC, hepatocellular carcinoma. (b) Bisulfite sequencing of the 5′ upstream region of rat Tslc1 gene. Sequence traces in NRL1 and HCC8 correspond to the genomic sequence (–127 to‐106 bp from the transcription initiation site) shown in the top line. CpG sites were numbers as 12, 13 and 14. Asterisks indicate methylated CpG sites.