Table 1.
Primer sequence | Annealing temperature (°C) | |
---|---|---|
Reverse transcription‐polymerase chain reaction | ||
Tslc1 | 1F: 5′‐TTATCCTCTGCAAGGCCTAAC‐3′ | 62 |
1R: 5′‐TGTTGAGGCATTTCGTCATC‐3′ | ||
Dal‐1 | 2F: 5′‐AGAGCCACAGAGGAATGACG‐3′ | 60 |
2R: 5′‐GGCACAGACCCCTAACATGA‐3′ | ||
Bisulfite sequencing | ||
Tslc1 | BS‐F: 5′‐GGGGAAGTAAAGGTTGAAATTTAA‐3′ | 60 |
BS‐R: 5′‐AACAACACAATACTCACCATAT‐3′ | ||
Dal‐1 | BS‐F: 5′‐TAGAGGATTTAGGTGTTTTAAAGA‐3′ | 56 |
BS‐R: 5′‐CTCAAAACCCCTCAACCACCAA‐3′ |