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Table 1.

The primer sequences used in this study

Primer sequence Annealing temperature (°C)
Reverse transcription‐polymerase chain reaction
Tslc1 1F: 5′‐TTATCCTCTGCAAGGCCTAAC‐3′ 62
1R: 5′‐TGTTGAGGCATTTCGTCATC‐3′
Dal‐1 2F: 5′‐AGAGCCACAGAGGAATGACG‐3′ 60
2R: 5′‐GGCACAGACCCCTAACATGA‐3′
Bisulfite sequencing
Tslc1 BS‐F: 5′‐GGGGAAGTAAAGGTTGAAATTTAA‐3′ 60
BS‐R: 5′‐AACAACACAATACTCACCATAT‐3′
Dal‐1 BS‐F: 5′‐TAGAGGATTTAGGTGTTTTAAAGA‐3′ 56
BS‐R: 5′‐CTCAAAACCCCTCAACCACCAA‐3′