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. 2007 Jul 23;98(10):1597–1603. doi: 10.1111/j.1349-7006.2007.00566.x

Figure 4.

Figure 4

Delta‐1 acts synergistically with FP6 on IL‐6Rα− early erythroid progenitors. (a) IL‐6Rα−Lin− cells (2500 cells/well) were cultured with 4GF, 4GF + IL‐6, 4GF + FP6, 4GF + FP6 + sgp130‐Fc (1 µg/mL) or 4GF + FP6 + IgG. The numbers of CD36+GPA+ cells were counted after 11–14 days of culture. Data are shown as the mean ± SD of triplicate cultures. (b) IL‐6Rα−Lin− cells (2500 cells/well) were cultured in liquid cultures with 4GF, 4GF + IL‐6 or 4GF + FP6 in the presence or absence of Delta‐1. The numbers of GPA+ cells were counted after 11–14 days of culture. Data are shown as the mean ± SD of triplicate cultures. *P < 0.05 compared to cells cultured with 4GF + FP6. (c) Quantitative real‐time RT‐PCR analysis of HES‐1, GATA‐2 and GATA‐1 gene expression levels in IL‐6Rα−Lin− cells and cells cultured for 24 h with 4GF + FP6 in the presence or absence of Delta‐1. Each gene expression level in cultured cells is expressed relative to the corresponding value in untreated cells. Data are shown as the mean ± SD of three independent experiments. *P < 0.05 compared to the gene expression level in cells cultured with 4GF + FP6.