Gene expression levels of drug efflux transporters of the ABC family by real‐time PCR in PC‐6 cells and pemetrexed (MTA)‐resistant cell lines, PC‐6/MTA‐1.6, PC‐6/MTA‐1.6‐2 and PC‐6/MTA‐4.0 cells. Comparison of ABCG2, ABCC1, ABCC2, ABCC3, ABCC5 and ABCC11 gene expression levels. The expression level of each gene was determined by real‐time PCR. **P < 0.0001.