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. 2010 Jul 27;101(11):2404–2410. doi: 10.1111/j.1349-7006.2010.01690.x

Figure 4.

Figure 4

 Modification of pemetrexed (MTA) cytotoxicity by ABCC11 siRNA. (a) Gene expression levels of ABCC11 in PC‐6 and PC‐6/MTA‐1.6 cells transfected with siRNA directed against ABCC11 (siABCC11), control siRNA‐transfected (siNEG) or non‐transfected PC‐6/MTA‐1.6 cells. **P < 0.0001. NS, not significantly different. (b) Cytotoxicity to MTA was measured in PC‐6 and PC‐6/MTA‐1.6 cells transfected with siRNA (siABCC11 or siNEG) or non‐transfected. Closed circle (•), PC‐6; closed triangle (), PC‐6/MTA‐1.6; open triangle (△), PC‐6/MTA‐1.6 transfected with ABCC11 siRNA; open circle (○), PC‐6/MTA‐1.6 transfected with negative‐control siRNA. (c) The intracellular methotrexate (MTX) concentration was measured in PC‐6/MTA‐1.6 cells transfected with siRNA (siABCC11 or siNEG) after exposure to MTX for 2 h. *P < 0.05.