Table 1.

Sequences of polymerase chain reaction primers and sequence‐specific probes for target genes and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH)

Gene	Forward primer	Reverse primer	Internal probe	Corresponding cDNA sequence
DCK	ATCCAGCTTCCTTCTGTCATTCC	CAACGAAGTGAGAGGCACCAG	CTCTTTTGTCTTCCTCAGCAGGTTGGCTT	1235–1314
CDA	AGGCAAGTCATGAGAGAGTTTGG	GCATTCTCTGGCTGTCACTGAGT	TGGCCCGTGTACATGACCAAGCC	424–572
DCD	CAGCACTGTTGGTGTTCGGA	GAAGGTGATGCTTGTGTAGGTGAA	CTCTTCTGTGCCCTGGCTCCATGC	1517–1586
ENT1	TCTTCATGGCTGCCTTTGC	GGCTTCACTTTCTTGGGCC	TCGCCAGCCTCTGCATGTGCTT	1295–1373
ENT2	CAAGACCTCATGGAAAGGGTG	CCACTCTGAACCCTCTGGTCA	CCCCACCACCAGGTCTGCATTTGT	1838–1961
NT5	TAATGGTATAAACACAGGATACCATCCT	CATTATCTACTACAGCTTGCTACCTGACT	TCTTGCAACACCCATGTGCCTTTGA	2457–2541
UCK2	GTGATCATCCCTAGAGGTGCAGATA	GGCCCTCCATTCAGGATGT	TCTGGTGGCCATCAACCTCATCGTG	634–716
GAPDH	GAAGGTGAAGGTCGGAGTC	GAAGATGGTGATGGGATTTC	CAAGCTTCCCGTTCTCAGCC	66–291

The probes were labeled with a reporter dye (FAM) situated at the 5′ end of the oligonucleotide and a quencher dye (TAMRA) located at the 3′ end.