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. 2009 Jul 23;100(11):2160–2166. doi: 10.1111/j.1349-7006.2009.01296.x

Figure 5.

Figure 5

 Interferon (IFN)‐γ secretion by macrophages and inhibitory effect of EL4 cells. (A) IFN‐γ production by cultured macrophages. After macrophages were cultured with dead EL4 (dEL4) cells and LPS for 24 h, IFN‐γ production in the supernatant was evaluated by ELISA (n = 4 for each group). *P < 0.05. (B) After macrophages were cultured with dEL4 cells and LPS for 8 h, expression of IFN‐γ mRNA was calculated by means of real‐time PCR. *P < 0.01. (C) EL4 cells were cultured with IFN‐γ for 6 h, and in vitro cytotoxicity was examined using the lactate dehydrogenase release assay. (D) EL4 cells were cultured with IFN‐γ for 24 h, and cell viability was evaluated using the WST assay.