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Fhl1 gene methylation in Src transformed cells. The transcriptional start site of Fhl1 (+1) lies in a CpG island of several hundred residues. PCR products from bisulfate‐treated DNA from non‐transformed and Src‐transformed cells were subcloned and sequenced. Data are shown as the percentage of each CpG sequence that was methylated (n = 5). More methylation of most of these sites was found in Src‐transformed cells than in non‐transformed cells.
