Figure 3.

RET knockdown abrogates glial cell line‐derived neurotrophic factor (GDNF)‐stimulated proliferation of non‐adherent NB‐39‐nu neuroblastoma cells. NB‐39‐nu cells (10 × 10⁴/mL, 2 mL/well) were seeded in conventional 6‐well plates in 10% fetal bovine serum containing RPMI‐1640 medium, followed by treatment with LacZ short interfering RNA (siRNA) or RET siRNA at 0 h and 24 h. At 48 h, the cells were harvested and seeded (3 × 10³/mL, 0.1 mL/well) in conventional or 2‐methacryloxyethyl phosphorylcholine‐treated 96‐well plates with or without GDNF (50 ng/mL) for 24 or 48 h. Cell proliferations were determined at the indicated time points. Columns, means of three replicates; bars, standard deviation.