Figure 4.
GDNF does not induce neuronal differentiation markers or morphological change in adherent NB‐39‐nu cells. NB‐39‐nu cells (10 × 104/mL, 2 mL/well) were seeded in conventional or 2‐methacryloxyethyl phosphorylcholine‐treated 6‐well plates in 10% fetal bovine serum containing RPMI‐1640 medium with or without glial cell line‐derived neurotrophic factor (GDNF; 50 ng/mL) for 24 or 48 h. (a) The cells were harvested for Western blot analysis at 24 h and 48 h. (b) Microscopic appearance of NB‐39‐nu cells cultured with or without GDNF under adherent or non‐adherent conditions at 48 h.