Figure 1.

  PCDH10 was silenced by hypermethylation in cervical cancer cells. (a) Among five cervical cancer cell lines (SiHa, CaSki, HeLa, HeLa3, and C33A), two human papillomavirus (HPV)‐immortalized human cervical epithelial cell lines (Z172 and Z183), and one immortalized keratinocyte (HaCaT) were examined by RT‐PCR. Only C33A expressed a significant amount of PCDH10. PCDH10 was weakly expressed or non‐expressed in the more invasive breast cancer cell line (MDA‐MB 231), melanoma cells (MDA‐MB 435), one of the three lung cancer cell lines (A549), and two ovarian cancer cell lines (OVCAR3 and SKOV3). (b) After treatment with 10 µM 5‐aza‐dC, PCDH10 was re‐expressed in SiHa, Caski, HeLa, and HeLaS3 cells. (c) Methylation‐specific‐PCR (MS‐PCR) confirmed the demethylation treatment. In contrast to little unmethylated (U) PCDH10 before treatment, both methylated (M) and U forms were found in these four cancer cells after demethylation treatment. Untreated white blood cell (WBC) DNA and H₂O were used as negative controls. The arrow signified the expected location of the PCR product.