Figure 1.

 (a,b) Nestin located in the cytoplasm in triple‐negative breast cancers. (c) Nestin expressed in myoepithelial cells. (d) Nestin protein expression in paired breast tissues was measured by western blotting. Protein (50 μL) from each sample was evaluated with expression of glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) as a loading control. Strong 177.439‐kD bands were detected in triple‐negative breast cancers (A1–4), whereas weaker or no bands were detected in other breast cancers (B1,2) or matching and adjacent non‐cancerous tissues (B3,4).