Figure 4.

 Simultaneous adsorption of DNA and generation of 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) on asbestos fibers. (a) Asbestos and silica adsorbed DNA after incubation at 37°C. Chrysotile was the most potent DNA adsorbent, followed by silica, crocidolite and amosite. *P < 0.05. **P < 0.01. (b) Crocidolite was incubated with genomic DNA at 4 or 37°C for 3 h. Fluorescent immunohistochemistry was performed after incubation to show the generation of 8‐OHdG on the asbestos surface (arrows). (c) Formation of 8‐OHdG on the surface of hemoglobin‐treated chrysotile after incubation with DNA in the presence of H₂O₂ (arrows). Scale bar, 50 μm.