Figure 4.
Effect of 67‐kDa laminin receptor (67LR) on urokinase‐type plasminogen activator (uPA), matrix metalloproteinase (MMP)‐9, and tissue inhibitor of matrix metalloproteinase (TIMP)‐1 in SGC7901 and MKN‐45 gastric cancer cell lines. (a,b) Expression of uPA, MMP2, and MMP9 in cytoplasm, and of active forms of MMP9 in supernatant. Tissue inhibitor of matrix metalloproteinase (TIMP)‐1 andTIMP2 were evaluated by western blot analysis. (c–f) Cells (2 × 105) were preincubated with immunoglobulin G (IgG; 1 μg/mL), uPA antibody (0.1 or 1 μg/mL), and MMP9 antibody (0.1 or 1 μg/mL) and then subjected to invasion assay, *P < 0.05; **P < 0.01 versus control and IgG treatment. Representative of three experiments with similar results.