Skip to main content
. 2010 Apr 19;101(7):1653–1660. doi: 10.1111/j.1349-7006.2010.01592.x

Figure 5.

Figure 5

 Hypoxia‐inducible factor 1 (HIF‐1)‐depen‐dent up‐regulation of 67‐kDa laminin receptor (67LR) by hypoxia. (a) Western blotting for HIF‐1 and 67LR protein expression under hypoxic (2, 4, 8 h) or normoxic conditions. (b) Dual‐immuno‐fluorescence staining against 67LR (green), HIF‐1 (red), or nuclear staining with DAPI (blue). Merged images are shown on the right in yellow. Magnification: ×40. (c) SGC7901 transiently trans‐fected with either a vector containing scrambled HIF‐1α, or a vector containing an HIF‐1α targeting sequence 48 h prior to incubation in normoxia or hypoxia for 8 h. Lysates were prepared and immunoblotted for HIF‐1α and 67LR. (d). Reverse transcription–PCR was used for analysis of 67LR mRNA levels in SGC7901 cells. Results shown are mean ± SE of three independent experiments performed in triplicate. *P < 0.05 versus SGC7901 in normoxia; #P < 0.05 versus SGC7901 transfected with scrambled sequence in hypoxia.