. 2011 Mar 14;102(6):1228–1235. doi: 10.1111/j.1349-7006.2011.01910.x

Table 1.

List of genes analyzed by semiquantitative RT‐PCR

Gene	Primer sequence (5′–3′)	Product size	PCR cycle number
SNAI1	TTTACCTTCCAGCAGCCCTA	199	33
SNAI1	CCTCATCTGACAGGGAGGTC	199	33
SNAI2	CTTTTTCTTGCCCTCACTGC	161	33
SNAI2	ACAGCAGCCAGATTCCTCAT	161	33
TWIST1	GGAGTCCGCAGTCTTACGAG	162	27
TWIST1	CCAGCTTGAGGGTCTGAATC	162	27
ZEB1	GCACAACCAAGTGCAGAAGA	190	27
ZEB1	CATTTGCAGATTGAGGCTGA	190	27
ZEB2	CGCTTGACATCACTGAAGGA	204	30
ZEB2	CTTGCCACACTCTGTGCATT	204	30
RHOA	TATCGAGGTGGATGGAAAGC	172	27
RHOA	TTCTGGGGTCCACTTTTCTG	172	27
CDC42	TACTGCAGGGCAAGAGGATT	172	27
CDC42	CCCAACAAGCAAGAAAGGAG	172	27
RAC1	TTTGAAAATGTCCGTGCAAA	250	24
RAC1	TCGCTTCGTCAAACACTGTC	250	24
GAPDH	ACCACAGTCCATGCCATCAC	452	27
GAPDH	TCCACCACCCTGTTGCTGTA	452	27