Figure 5.

 Silencing of the NEU3 gene in melanomas resulted in definite suppression of enzyme activities and cell growth. (A,B) A HeLa cell was used to confirm the silencing effects of neu3‐s3 siRNA, showing 75% reduction in relative mRNA levels (A), and significant reduction in BrdU uptake (B). (C) To knockdown human NEU3 gene, a siRNA, neu3‐s3 was used. The siRNA GL2 was used as a negative control. Experiments repeated at least three times showed similar results. (D) NEU3 sialidase activity (substrate: GM3) in the NEU3‐silenced cells was examined. (E,F) Suppression of cell proliferation with gene silencing of NEU3 in MeWo. Silencing effects of NEU3 mRNA in MeWo (E). Cell proliferation was assayed with BrdU uptake after transfection with neu3‐s3 siRNA, showing significant suppression in repeated experiments (F).