Figure 10.
Effect of hScrib on the activity of the APC‐cyclin D pathway. Cell lysates generated from NIH3T3 cells transfected with GFP or each GFP‐fusion hScrib expression clones were examined by Western blotting with an anti‐APC antibody, anticyclin A antibody, anticyclin D1 antibody. An antitubulin antibody was used to equilibrate loading for each lane. Cell lysates from GFP‐fusion hScrib expression clones that have its LRRs and at least one complete PDZ domain expressed 5–7‐fold less cyclin A and D1 than lysates from GFP‐transfected cells. In contrast, LRR + PDZ 1b1+747 in which the conserved hydrophobic pocket was partially deleted had no effect on the regulation of cyclins A and D1.