Figure 11.

(A) NIH3T3 cells transfected with GFP only, GFP‐hScrib(FL), mutant hScrib were analyzed by flow cytometry to examine the effects of hScrib on cell‐cycle progression. Gates were established such that only cells with high GFP fluoresce. Note that LRR + PDZ 1–2 GRGI, a mutant in which the conserved Gly‐Leu‐Gly‐Ile pocket motif in PDZ domain 1 was replaced with Gly‐Arg‐Gly‐Ile, has no effect on cell‐cycle regulation of G1/S transition. (B) The approximate percentages of cells in each of the major phases of the cell cycle were calculated using the Multicycle software. The average difference in the percentage of cells (Y‐axis) in each phase of the cell cycle (X‐axis) among GFP‐hScrib mutants from three different experiments is shown. Percentages of cells transfected with GFP as a control were set to zero in the three phases (G1, S, and G2/M), and changes of cell‐cycle distribution were shown for cells transfected with GFP‐hScrib full‐length and mutants.