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. 2006 Sep 5;97(11):1217–1225. doi: 10.1111/j.1349-7006.2006.00315.x

Figure 9.

Figure 9

BrdU incorporation analysis in NIH3T3 cells transfected with pEGFP‐hScrib expression clones. Cell‐cycle regulatory function of hScrib was analyzed by the rate of BrdU incorporation in serum‐starved NIH3T3 cells transfected with pEGFP‐hScrib expression clones. a. Immunofluorescence image of GFP‐fused hScrib domain construct. b. Immunofluorescence image of BrdU‐incorporated cells. c. Merged image of a. and b. NIH3T3 cells were transfected with (A) pEGFP vector, (B) pEGFP‐hScrib expression plasmid, (C) pEGFP‐hScrib LRR + PDZ 1–2 expression plasmid, (D) pEGFP‐hScrib LRR + PDZ 1–2 GRGI, which was a mutant in which the conserved Gly‐Leu‐Gly‐Ile pocket motif in PDZ domain 1 was replaced with Gly‐Arg‐Gly‐Ile expression plasmid, (E) pEGFP‐hScrib LRR expression plasmid, and (F) pEGFP‐hScrib PDZ 1–4 expression plasmid.