Figure 1.

 Experimental paradigm design. (a) Formalin‐fixed paraffin‐embedded (FFPE) samples were cored with a 3‐mm diameter needle and arranged in a line with three cancer tissues and one adjacent normal tissue. The histological type of the cancer of Patient 1 was moderately differentiated adenocarcinoma, that of Patient 2 was well‐differentiated adenocarcinoma, and that of Patient 3 was poorly differentiated adenocarcinoma. Hematoxylin–eosin stain, ×10. Scale bar, 1 mm. Enclosed area corresponded to magnified microscopic image. Hematoxylin–eosin stain, ×400 (b) The schema of FFPE samples and the workflow of statistical analysis are shown. (c) The schema that categorizes the acquired signals is presented.