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. 2008 Jan 15;99(3):615–622. doi: 10.1111/j.1349-7006.2007.00723.x

Figure 2.

Figure 2

Inhibitory effects of RelA‐associated inhibitor (RAI) on p53‐ and p65‐dependent gene expression. (a) A total of 293 cells were transfected with bax‐luc together with pC53‐SN3 and various amounts of pFLAG‐CMV2‐RAI. (b) Saos2 cells were transfected with p21‐luc together with pC53‐SN3 and various amounts of pFLAG‐CMV2‐RAI. (c) A total of 293 cells were transfected with κB‐luc together with pGL3‐p65 and various amounts of pFLAG‐CMV2‐RAI. The luciferase activity was normalized by Renilla luciferase activity, which was cotransfected as an internal control. The data are represented as the fold increase in luciferase activities (mean ± SD) relative to control transfection of three independent experiments.