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. 2009 Aug 27;100(12):2419–2425. doi: 10.1111/j.1349-7006.2009.01332.x

Figure 7.

Figure 7

 Pigment epithelium‐derived factor (PEDF) down‐regulates hypoxia‐induced factor (HIF)‐1α in retinoblastoma cells and xenografted tissues. Immunocytochemistry analysis of HIF‐1α expression in SO‐Rb 50 cells (a,b). SO‐Rb50 cells were incubated with or without PEDF (100 nmol/L) for 10 h under normoxia and hypoxia (×640). HIF‐1α protein level highlighted by immunocytochemistry. a1, a2, a3, a4 correspond to N, N + P, H, and H + P in graph B, respectively. Values significantly lower than controls (*P < 0.05, **P < 0.01, #P < 0.05, ##P < 0.01, *vs group N, # vs group H) are indicated. Western blotting analysis for HIF‐1α in xenografted tumor tissues (c,d). Values significantly lower than control (**P < 0.01) are indicated.