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. 2009 Mar 11;100(5):859–865. doi: 10.1111/j.1349-7006.2009.01115.x

Figure 1.

Figure 1

Tumor cells prefer to adhere to fibrinogen than to platelets. (A) One hundred microliters of 2 mg/mL fibrinogen (Alexa Fluor488 conjugated, green) or 2 × 108/mL platelets (PKH26 dyed, red) were incubated with 100 µL of 2 × 105 YAC‐1 or B16F10 cells in phosphate‐buffered saline (PBS) containing Ca2+ (sample) or ethylenediaminetetraacetic acid (control) for 30 min. Tumor cells adhered to fibrinogen or platelets were counted by fluorescence‐activated cell sorting before and after washing three times with PBS. FS, Forward Scatter; SS, Side Scatter. (B) Statistical results (control and washed samples) from flow cytometry. (C) Fibrinogen or platelet monolayers on glass slides were washed with PBS/0.1% bovine serum albumin for 2 min, tumor cells (1 × 106/mL) were perfused over the layers at the shear stress of 1.2 dyn/cm2, and the number of tumor cells adhered to fibrinogen or platelets were counted in 10 randomly selected fields after 3 min. All of the experiments were repeated more than three times. **P < 0.01; ***P < 0.001.