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. 2008 Oct 21;100(1):150–157. doi: 10.1111/j.1349-7006.2008.00996.x

Figure 1.

Figure 1

Effect of phthoxazolin A (Phx A) on cocultures of DU‐145 cells and prostate stromal cells (PrSC). (a) Effects of Phx A and inthomycin B on the growth of DU‐145 cells alone (open circles), DU‐145 cells co‐cultured with PrSC (closed red circles), or PrSC alone (closed blue squares) were determined using rhodanile blue staining. Values represent means of duplicate determinations (SE < 10%). (b) Representative photos of DU‐145 cells cocultured with PrSC (co), DU‐145 cells cultured alone (DU‐145), and PrSC cultured alone (PrSC) after staining with rhodanile blue. Cells were treated with the indicated concentrations of Phx A. Scale bar = 200 µm. (c) PrSC were precultured with Phx A for the indicated times. Cultured conditioned medium (CM) was collected, and DU‐145 cells were cultured in the CM for 3 days. Values represent means of duplicate determinations (SE < 10%). All data are representative of three independent experiments with similar results.