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. 2008 Oct 21;100(1):150–157. doi: 10.1111/j.1349-7006.2008.00996.x

Figure 3.

Figure 3

Two‐dimensional gel analysis of prostate stromal cell (PrSC)‐conditioned medium (CM). PrSC were cultured with 50 µg/mL phthoxazolin A (Phx A) for 3 days. Ammonium sulfate precipitates of the CM were analyzed by two‐dimensional gel analysis. (a) The representative photo of the gels and protein spots decreased by Phx A (red) and increased by Phx A (blue). (b) Ammonium sulfate precipitates of the CM were separated by sodium dodecylsulfate–polyacrylamide gel electrophoresis and immunoblotted with anti‐insulin‐like growth factor binding protein (IGFBP)‐5 antibodies. IGFBP‐5 protein was detected as a doublet of 33–35 kDa.( 38 ) TPT, tumor protein, translationally‐controlled.