Figure 5.

Effect of phthoxazolin A (Phx A) on transforming growth factor (TGF)‐β signaling. (a) Prostate stromal cells (PrSC) were cultured with Phx A or SB431542 (SB) in the presence of 10 ng/mL TGF‐β1 for 1 day. Total RNA was collected and reverse transcription–polymerase chain reaction for the indicated molecules was carried out using specific primers. (b) PrSC were pretreated with Phx A or SB for 30 min, and then treated with 10 ng/mL TGF‐β1 for 30 min. Cell lysates were prepared and used for western blotting. All data are representatives of three independent experiments with similar results. GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; IGF, insulin‐like growth factor; IGFBP, IGF binding protein.