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. 2008 Oct 21;100(1):150–157. doi: 10.1111/j.1349-7006.2008.00996.x

Figure 6.

Figure 6

Inhibition of insulin‐like growth factor (IGF)‐I activity in prostate stromal cell (PrSC)‐conditioned medium (CM) by phthoxazolin A (Phx A). (a) PrSC were cultured with Phx A for 2 days and the amounts of IGF‐I in PrSC‐CM were measured. Values are means ± SD for triplicate determinations. **p<0.02 versus control. (b) CM was prepared from PrSC cultured with the indicated concentrations of Phx A for 2 days (PrSC/Phx A). DU‐145 cells were treated with CM or normal medium for 30 min (medium changed). Cell lysates were prepared and used for immunoprecipitation of IGF‐IR for the detection of tyrosine‐phosphorylated IGF‐IR and total IGF‐IR, or directly for the detection of phosphorylated Akt and total Akt. (c) PrSC were precultured with Phx A for 2 days, and then DU‐145 cells were cocultured with the PrSC by the external addition of IGF‐I at 500 ng/mL. Values are means ± SD for triplicate determinations. All data are representative of three independent experiments with similar results.