Table 4.

Sensitivity and specificity of the system

	Sensitivity	Specificity
PNA‐LNA PCR clamp for mutations with detection probes	1.00	1.00 ‡
PNA‐LNA PCR clamp for all mutations	>0.98 †	1.00 ‡
Cancer cell content is more than 1%	0.99 §	1.00 ¶
Overall	>0.97	1.00

^† Our system detects 267 out of 269 mutation occurrences listed in Fig. 2C. Step 3 detects 240 occurrences (89%). Step 4 detects additional 27 occurrences (10%). Two occurrences which failed to be detected are those of G719A.

^{‡ ‡} More than 200 controls did not demonstrate any false results.

^§ At Step 2, 59 of 59 specimens had a cancer content of more than 1% (see Fig. 4).

^{¶ ¶} Specificity of the pathological examination is assumed to be 1.0 in this study. PNA‐LNA PCR, peptide nucleic acid‐locked nucleic acid polymerase chain reaction.