Figure 6.
Analysis of the hypoxia‐inducible factor 1 (HIF‐1) binding to the hypoxia responsive element (HRE) motif of the S100A4 gene in vivo by ChIP assay. BGC823 cells were grown under normoxic or hypoxic conditions for 24 h. Lysates of BGC823 cells were immunoprecipitated with HIF‐1α antibody or normal rabbit IgG. Subsequently, a DNA segment of the first intron of the S1004 gene containing the HRE was amplified by PCR. Lysates without immunoprecipitation were used as the control for DNA input in each sample. Binding of HIF‐1α was increased by CoCl2 treatment compared with untreated samples.