Figure 1.

WM‐266‐4 cells can grow as spheroids in serum‐depleted culture conditions. (a) WM‐266‐4 cell morphology in normal culture conditions with 10% fetal bovine serum is shown. Typical mesenchymal cells with two or three cytoplasmic projections and long cell body are attached. Bar = 25 µm. (b) Fifteen days after medium change into neural stem‐cell culture conditions, most cell aggregates disappeared as cell debris, and small portions could grow as spheroids. Inset box shows a representative spheroid that has a dark central shadow and is over 150 µm in diameter. Bar = 100 µm.