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. 2009 Dec 9;101(4):969–974. doi: 10.1111/j.1349-7006.2009.01463.x

Figure 3.

Figure 3

  In vitro antitumor activity of 22A31. (a) Direct effect of 22A31 on ACC‐MESO‐4 tumor cell growth. After 72 h of incubation with indicated concentration of 22A31 (black bar) or normal mouse IgG1k (white bar), XTT assay was performed and absorbance was presented. (b) 22A31‐induced complement‐dependent cytotoxicity (CDC). %Cyto‐toxicity index (C.I.) of the indicated concentrations of 22A31 or normal mouse IgG1k against ACC‐MES‐4 tumor cells are indicated. (c,d) 22A31‐inducd antibody‐dependent cell mediated cytotoxicity (ADCC) against ACC‐MESO‐4 tumor cells (c) or NCI‐H226 tumor cells (d) with purified natural killer (NK) cells. ADCC induced by 1 μg/mL of 33A31 or normal mouse IgG1κ was examined at indicated effector/target ratios when purified NK cells were used as effector cells. (e) 22A31‐inducd ADCC against C‐ERC/mesothelin‐negative Huh7 tumor cells with purified NK cells. ADCC induced by 1 μg/mL of 33A31 or normal mouse IgG1κ was examined at indicated effector/target ratios when purified NK cells were used as effector cells. (f) 22A31‐inducd ADCC against ACC‐MESO‐4 tumor cells with purified macrophages. ADCC induced by 1 μg/mL of 33A31 or normal mouse IgG1κ was examined at indicated effector/target ratios when purified macrophages were used as effector cells. Asterisk indicates statistical significance.