Figure 2.

Sensitivity of tumor cells to treatment with the Escherichia coli purine nucleoside phosphorylase gene and 6‐methoxypurine 2′‐deoxyriboside (ePNP/MoPdR). (a) The infected murine mammary carcinoma 4T1 cells were treated with MoPdR concentrations ranging from 0.05 to 10 µg/mL. The absorbance of each well at a wavelength of 570 nm was measured using an enzyme‐linked immunosorbent assay reader (Bio‐Rad) 96 h later. (b) At the concentration of 5 µg/mL MoPdR, cell survival was quantified at indicated time points by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide assay. Data are representative of at least three separate experiments; each point represents the mean ± SE and is expressed as a percentage relative to the same cells non‐treated. SC/ePNP, triangle; SC/pEGFP, square.