Figure 5.
Bacterial distribution in murine tissues. (a) Preferential accumulation of salmonella in the tumors of mice given S. typhimurium carrying pEGFP‐c1‐ePNP (SC/ePNP). Mice bearing mammary carcinoma 4T1 cells were given 1 × 109 c.f.u. of SC/ePNP, orally. The amounts of SC/ePNP accumulated in tumors, livers, spleens, kidneys, and gastrointestinal tracts were determined at 1, 7, 12, and 16 days post‐infection. Each value represents the mean ± SD from three mice. n.d., not detected. (b) Reverse transcription–polymerase chain reaction (RT‐PCR) analysis for gene expression. At different time points after bacterial inoculation (7 and 12 days), the tissues of mice bearing mammary carcinoma 4T1 tumors were tested for ePNP expression by RT‐PCR. M, molecular marker profile.